CCR2a-specific Rekombinanter Antikörper

CCR2a-specific Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242217G4

Kat-Nr. : 84911-3-RR

Synonyme

CCR2, 242217G4, C C chemokine receptor type 2, C C CKR 2, C-C chemokine receptor type 2

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  WB analysis using 84911-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84911-3-RR (CCR2a-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 84911-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84911-3-RR (CCR2a-specific antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 84911-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84911-3-RR (CCR2a-specific antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 84911-3-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CCR2a-specific antibody (84911-3-RR, Clone: 242217G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, A431-Zellen, Jurkat-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84911-3-RR bindet in WB, IF/ICC, ELISA CCR2a-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: chemokine (C-C motif) receptor 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_000647
• Gene symbol: CCR2
• Gene ID (NCBI): 729230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates.