CCR7 Polyklonaler Antikörper

CCR7 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 25898-1-AP

Synonyme

BLR2, C C chemokine receptor type 7, C C CKR 7, CC CKR 7, CCR 7, CCR7, CD197, CDw197, CMKBR7, EBI1, EVI1, MIP 3 beta receptor

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Galerie der Validierungsdaten

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  WB analysis using 25898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25898-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 25898-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 25898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25898-1-AP (CCR7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 25898-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 25898-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25898-1-AP (CCR7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, C6-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

25898-1-AP bindet in WB, IHC, IF, ELISA CCR7 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCR7 fusion protein Ag22941
• Vollständiger Name: chemokine (C-C motif) receptor 7
• Berechnetes Molekulargewicht: 378 aa, 43 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC035343
• Gene symbol: CCR7
• Gene ID (NCBI): 1236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-C chemokine receptor type 7 (CCR7), which belongs to the G-protein coupled receptor 1 family, is also a receptor for the MIP-3-beta chemokine, and probably acts as a mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. CCR7 is specially expressed in various lymphoid tissues and activated B- and T-lymphocytes, and can be strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

Publikationen

Species	Application	Title
rat	IF	Bioact Mater; Two-stage degradation and novel functional endothelium characteristics of a 3-D printed bioresorbable scaffold.; Authors - Tieying Yin
mouse	IHC	Virulence; Intranasal bovine β-defensin-5 enhances antituberculosis immunity in a mouse model by a novel protein-based respiratory mucosal vaccine.; Authors - Zhengmin Liang
rat	IHC	Front Cell Dev Biol; Potential Mechanisms of the Impact of Hepatocyte Growth Factor Gene-Modified Tendon Stem Cells on Tendon Healing.; Authors - Mingzhao Zhang
rat	IF	Stem Cell Res Ther; Tendon stem cell-derived exosomes regulate inflammation and promote the high-quality healing of injured tendon.; Authors - Mingzhao Zhang
human	IHC	Front Mol Biosci; CCR7 Has Potential to Be a Prognosis Marker for Cervical Squamous Cell Carcinoma and an Index for Tumor Microenvironment Change.; Authors - Wei-Jie Tian
human	WB	Front Pharmacol; MicroRNA-532-3p Suppresses Malignant Behaviors of Tongue Squamous Cell Carcinoma via Regulating CCR7.; Authors - Cuijuan Feng