CCRL2 Monoklonaler Antikörper

CCRL2 Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2C1E11

Kat-Nr. : 66611-1-Ig

Synonyme

C C chemokine receptor like 2, CCR11, CCR6, CCRL2, Chemokine receptor CCR11, Chemokine receptor X, CKRX, CRAM, CRAM A, CRAM B, HCR

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 66611-1-Ig

  HeLa cells and A431 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 66611-1-Ig

  A431 cells and K-562 cells were subjected to SDS PAGE followed by western blot with 66611-1-Ig (CCRL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 66611-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 66611-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66611-1-Ig (CCRL2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

66611-1-Ig bindet in WB, IHC, ELISA CCRL2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CCRL2 fusion protein Ag4045
• Vollständiger Name: chemokine (C-C motif) receptor-like 2
• Berechnetes Molekulargewicht: 344 aa, 40 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC025717
• Gene symbol: CCRL2
• Gene ID (NCBI): 9034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chemokine CC motif receptor-like 2 (CCRL2) is a seven-transmembrane domain receptor that shares structural and functional similarities with the family of atypical chemokine receptors (ACKRs) (PMID: 28743719). CCRL2 does not appear to be a signaling receptor, but may have a role in modulating chemokine-triggered immune responses by capturing and internalizing CCL19 or by presenting RARRES2 ligand to CMKLR1, a functional signaling receptors. CCRL2 has been found on almost all hemopoietic cells, including monocytes, macrophages, polymorphonuclear neutrophils (PMNs), T cells, dendritic cells, natural killer cells, and CD34+ progenitor cells, with PMNs expressing the highest levels (PMID: 15188357). CCRL2 is also expressed by barrier cells, such as lymphatic and blood endothelial cells and bronchial and intestinal epithelial cells (PMID: 29056935).

Publikationen

Species	Application	Title
human	WB	PLoS Pathog; Identification of viral SIM-SUMO2-interaction inhibitors for treating primary effusion lymphoma.; Authors - Ling Ding
human	WB	Onco Targets Ther; Oncogenic Role and Prognostic Value of MicroRNA-937-3p in Patients with Breast Cancer.; Authors - Deyu Li
human	WB	Front Immunol; Identification and validation of chemokine system-related genes in idiopathic pulmonary fibrosis; Authors - Tianming Zhao