CCT2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CCT2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (2)

Kat-Nr. : 24896-1-AP

Synonyme

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

---

• 

  WB analysis of mouse testis using 24896-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24896-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24896-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 24896-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24896-1-AP (CCT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 24896-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CCT2 antibody (24896-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

24896-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CCT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCT2 fusion protein Ag19264
• Vollständiger Name: chaperonin containing TCP1, subunit 2 (beta)
• Berechnetes Molekulargewicht: 535 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-58 kDa
• GenBank-Zugangsnummer: BC113516
• Gene symbol: CCT2
• Gene ID (NCBI): 10576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)

Publikationen

Species	Application	Title
human	WB, IF	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
human	WB	Pharmacol Res; Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.; Authors - Jiale Zhu ; KD Validated
human	WB	PLoS One; Proteomic analysis of mesenchymal stem cells from normal and deep carious dental pulp.; Authors - Dandan Ma
mouse	IF	Signal Transduct Target Ther; Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease; Authors - Boyuan Ma
human	WB	Autophagy; Copper-dependent autophagic degradation of GPX4 drives ferroptosis; Authors - Qian Xue
human	WB	Mol Cell; Genome-scale exon perturbation screens uncover exons critical for cell fitness; Authors - Mei-Sheng Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Rashmi (Verified Customer) (02-14-2025)

Used for Western Blot, Bands are clearly visible.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 , 1:2000
• Cell Tissue Type: HEK293T

FH

Rashmi (Verified Customer) (02-04-2025)

Used for Western blot, Highly Recommended

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293