CCT2 Polyklonaler Antikörper

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• KD/KO Validated

CCT2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24896-1-PBS

Synonyme

PRO1633, Chaperonin containing T-complex polypeptide 1 subunit 2, CCT-beta, CCTB, CCT beta

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  WB analysis of mouse testis using 24896-1-AP (same clone as 24896-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 24896-1-AP (same clone as 24896-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24896-1-AP (CCT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 24896-1-AP (same clone as 24896-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 24896-1-AP (CCT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 24896-1-AP (same clone as 24896-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CCT2 antibody (24896-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24896-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24896-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CCT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCT2 fusion protein Ag19264
• Vollständiger Name: chaperonin containing TCP1, subunit 2 (beta)
• Berechnetes Molekulargewicht: 535 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-58 kDa
• GenBank-Zugangsnummer: BC113516
• Gene symbol: CCT2
• Gene ID (NCBI): 10576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT2 is a gene encoding a molecular chaperone that is a member of the chaperonin-containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). The switch of CCT2ʹs role from a chaperonin to a specific aggrephagy receptor is achieved by CCT2 monomer formation. CCT2 functions independently of ubiquitin and the TRiC complex to facilitate the autophagic clearance of solid protein aggregates. CCT2 has 2 isoforms with a molecular mass of 53 and 57 kDa. (PMID: 35699934)