CCT4 Monoklonaler Antikörper

CCT4 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C6A6

Kat-Nr. : 67455-1-PBS

Synonyme

CCT DELTA, CCT4, Cctd, SRB, Stimulator of TAR RNA binding, TCP 1 delta

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  WB analysis using 67455-1-Ig (same clone as 67455-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67455-1-Ig (CCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67455-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67455-1-Ig (same clone as 67455-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT4 antibody (67455-1-Ig, Clone: 3C6A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67455-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67455-1-PBS bindet in WB, IF/ICC, Indirect ELISA CCT4 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CCT4 fusion protein Ag17084
• Vollständiger Name: chaperonin containing TCP1, subunit 4 (delta)
• Berechnetes Molekulargewicht: 539 aa, 58 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC106934
• Gene symbol: CCT4
• Gene ID (NCBI): 10575
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

T-complex protein 1 subunit delta (CCT4) is a component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PMID:25467444). As part of the TRiC complex, it may play a role in regulating telomere maintenance (PMID:25467444) and assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PMID:20080638). CCT4 has 2 isoforms produced by alternative splicing with the MW of 55 kDa and 58 kDa.