CCT5 Rekombinanter Antikörper

CCT5 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241510B3

Kat-Nr. : 84310-2-PBS

Synonyme

241510B3, CCT epsilon, CCTE, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

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  WB analysis using 84310-2-RR (same clone as 84310-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84310-2-RR (CCT5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84310-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01209-1

  The mean CCT5 concentration was determined to be 28.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load and 35.60 ng/mL in HEK-293 cell extract based on a 2.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01209-1

  Sandwich ELISA standard curve of MP01209-1, Human CCT5 Recombinant Matched Antibody Pair - PBS only. 84310-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2146. 84310-1-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

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  IF Staining of HepG2 using 84310-2-RR (same clone as 84310-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCT5 antibody (84310-2-RR, Clone: 241510B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84310-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01209-1

  Cytometric bead array standard curve of MP01209-1, CCT5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84310-2-PBS. Detection antibody: 84310-1-PBS. Standard: Ag2146. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 84310-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 84310-2-PBS against Human CCT5 was performed. The affinity constant is below 1 pM.

• 

  Affinity and Kinetic Characterization of 84310-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84310-2-RR against Human CCT5 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84310-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA CCT5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CCT5 fusion protein Ag2146
• Vollständiger Name: chaperonin containing TCP1, subunit 5 (epsilon)
• Berechnetes Molekulargewicht: 541 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC035499
• Gene symbol: CCT5
• Gene ID (NCBI): 22948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT5 is a subunit of chaperonin containing TCP1 complex that is also known as the TCP1 ring complex (TRiC).CCT5 involves enhancing Wnt/β-catenin signalling activity in GCs through abrogating the interaction between E-cadherin and β-catenin (PMID: 35194191). The expression levels of CCT5 have been found to be upregulated in types of cancers, including breast cancer, colon cancer, lung cancer, glioblastoma, hepatocellular carcinoma and ovarian cancer (PMID: 36768350). Human CCT5 consist of 541 amino acids organized in β-sheets and α-helixes distributed into three domains and has a predicted molecular weight of 60 kDa.