CCT7 Polyklonaler Antikörper

CCT7 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 15994-1-AP

Synonyme

CCT ETA, CCT7, Ccth, HIV 1 Nef interacting protein, Nip7 1, TCP 1 eta

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15994-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15994-1-AP (CCT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15994-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15994-1-AP (CCT7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15994-1-AP

  IP Result of anti-CCT7 (IP:15994-1-AP, 4ug; Detection:15994-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

• 

  IF Staining of MCF-7 using 15994-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCT7 antibody 15994-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15994-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CCT7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CCT7 fusion protein Ag8868
• Vollständiger Name: chaperonin containing TCP1, subunit 7 (eta)
• Berechnetes Molekulargewicht: 543 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC019296
• Gene symbol: CCT7
• Gene ID (NCBI): 10574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.

Publikationen

Species	Application	Title
human	WB	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
	WB	Nat Commun; Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.; Authors - Adela S Oliva Chávez
human	IHC	Aging (Albany NY); Overexpression of chaperonin containing TCP1 subunit 7 has diagnostic and prognostic value for hepatocellular carcinoma.; Authors - Xinghua Huang
mouse	WB	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal
human	WB	Nat Commun; Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.; Authors - Erika C Urdaneta
human	WB	Clin Proteomics; Quantitative proteomics identified circulating biomarkers in lung adenocarcinoma diagnosis; Authors - Hongyu Chen