TCP1 eta Monoklonaler Antikörper

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TCP1 eta Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C1G1

Kat-Nr. : 67540-1-PBS

Synonyme

CCT7, CCT ETA, CCT-eta, CCTH, Chaperonin containing T-complex polypeptide 1 subunit 7

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  WB analysis using 67540-1-Ig (same clone as 67540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67540-1-Ig (same clone as 67540-1-PBS)

  WB result of CCT7 antibody (67540-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67540-1-Ig (same clone as 67540-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67540-1-Ig (CCT7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67540-1-Ig (TCP1 eta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67540-1-Ig (same clone as 67540-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCT7 antibody (67540-1-Ig, Clone: 2C1G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67540-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67540-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA TCP1 eta und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TCP1 eta fusion protein Ag9502
• Vollständiger Name: chaperonin containing TCP1, subunit 7 (eta)
• Berechnetes Molekulargewicht: 543 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC019296
• Gene symbol: CCT7
• Gene ID (NCBI): 10574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCT7 is a subunit of CCT complex-a large cytosolic chaperonin complex composed of two hetero-oligomeric stacked rings able to interact with nascent polypeptides, which mediates protein folding in an ATP-dependent manner and prevents aggregation in eukaryotes. Each ring consists of eight different subunits (CCT1 to CCT8). CCT7 is involved in preventing aggregation of GPCRs and in regulating their expression, maturation, and transport to the cell surface.