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CCT8 Polyklonaler Antikörper
CCT8 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 12263-1-AP
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, COLO 320-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausnierengewebe, Raji-Zellen |
| Erfolgreiche IP | Jurkat-Zellen |
| Erfolgreiche Detektion in IF/ICC | SW480-Zellen, MCF-7-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Produktinformation
12263-1-AP bindet in WB, IF/ICC, IP, ELISA CCT8 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | CCT8 fusion protein Ag2926 |
| Vollständiger Name | chaperonin containing TCP1, subunit 8 (theta) |
| Berechnetes Molekulargewicht | 548 aa, 60 kDa |
| Beobachtetes Molekulargewicht | 60 kDa |
| GenBank-Zugangsnummer | BC012584 |
| Gene symbol | CCT8 |
| Gene ID (NCBI) | 10694 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for CCT8 antibody 12263-1-AP | Protokoll herunterladen |
| IF protocol for CCT8 antibody 12263-1-AP | Protokoll herunterladen |
| IP protocol for CCT8 antibody 12263-1-AP | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Nat Commun Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan. | ||
Proc Natl Acad Sci U S A BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rashmi (Verified Customer) (02-14-2025) | Used for Western Blot, Bands are clear
|
FH Rashmi (Verified Customer) (02-04-2025) | Used for Western blot, Highly Recommended
|
FH Joleen (Verified Customer) (06-03-2019) | CCT8 band shows up prominently with no background.
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