MME,CD10 Polyklonaler Antikörper

MME,CD10 Polyklonal Antikörper für FC, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23782-1-AP

Synonyme

Atriopeptidase, CALLA, CD10, Enkephalinase, EPN, membrane metallo endopeptidase, MME, MME,CD10, NEP, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using MME,CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using MME,CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 23782-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human MME,CD10 (23782-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Nierengewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23782-1-AP bindet in IHC, IF, FC, ELISA MME,CD10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MME,CD10 fusion protein Ag20634
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• GenBank-Zugangsnummer: BC101632
• Gene symbol: MME
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MME is also names as neprilysin,CALLA,NEP,SFE,CD10 and belongs to the peptidase M13 family.It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium.CD10 has been considered a useful marker in the diagnosis of renal carcinomas, because of its expression in clear cell and papillary renal cell carcinomas and its absence in chromophobe renal cell carcinomas(PMID:15286660).