Neprilysin/CD10 Polyklonaler Antikörper

Neprilysin/CD10 Polyklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 23782-1-AP

Synonyme

MME, MME,CD10, EPN, Enkephalinase, EC:3.4.24.11

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  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23782-1-AP (Neprilysin/CD10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 23782-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 23782-1-AP (CD10,MME Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human kidney using 23782-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Neprilysin/CD10 antibody (23782-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Nierengewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

23782-1-AP bindet in IHC, IF-P, ELISA Neprilysin/CD10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Neprilysin/CD10 fusion protein Ag20634
• Vollständiger Name: membrane metallo-endopeptidase
• Berechnetes Molekulargewicht: 750 aa, 86 kDa
• GenBank-Zugangsnummer: BC101632
• Gene symbol: CD10
• Gene ID (NCBI): 4311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD10, also known as neprilysin, membrane metallo-endopeptidase (MME), neutral endopeptidase (NEP), or common acute lymphoblastic leukemia antigen (CALLA), is a 100-kDa type II transmembrane glycoprotein belonging to peptidase M13 family (PMID: 7760013; 8102558). Among hematopoietic cells, CD10 is expressed on granulocytes, B cell precursors, mature germinal center B cells, a subset of immature thymocytes (PMID: 13679451). CD10 is also expressed on a variety of nonhematopoietic cell types, including bronchial epithelial cells, cultured fibroblasts, bone marrow stromal cells, renal proximal tubular epithelial cells, breast myoepithelium, biliary canaliculi (PMID: 8102558). CD10 is a cell surface peptidase that cleaves peptide bonds on the amino side of hydrophobic amino acids and inactivates a variety of physiologically active peptides. Loss or decreases in CD10 expression have been reported in a variety of malignancies (PMID: 16054017).

Publikationen

Species	Application	Title
mouse	IF	Clin Breast Cancer; Inhibitory Impact Of Cinobufagin In Triple-Negative Breast Cancer Metastasis: Involvements Of Macrophage Reprogramming Through Upregulated MME and Inactivated FAK/STAT3 Signaling; Authors - Zhaohui Zhu