CD100 Polyklonaler Antikörper

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CD100 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27516-1-PBS

Synonyme

SEMA4D, A8, CD 100, CD100 antigen, SEM4D

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  WB analysis using 27516-1-AP (same clone as 27516-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27516-1-AP (CD100 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 27516-1-AP (same clone as 27516-1-PBS)

  WB result of CD100 antibody (27516-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD100 transfected HeLa cells. This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 27516-1-AP (same clone as 27516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27516-1-AP (CD100 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27516-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27516-1-PBS bindet in WB, IHC, Indirect ELISA CD100 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD100 fusion protein Ag26461
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: BC054500
• Gene symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.