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CD100 Rekombinanter Antikörper

CD100 Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241708D10

Kat-Nr. : 84465-4-PBS

Synonyme

SEMA4D, SEM4D, CD100 antigen, CD 100, A8

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using CD100 Recombinant antibody (84465-4-RR)

    IF Staining of Jurkat using 84465-4-RR (same clone as 84465-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD100 antibody (84465-4-RR, Clone: 241708D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84465-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84465-4-RR

    Affinity and Kinetic Characterization of 84465-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84465-4-RR against Human CD100 were performed. The affinity constant is 3.21 nM.

Geprüfte Anwendungen

Produktinformation

84465-4-PBS bindet in IF/ICC, Indirect ELISA CD100 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Berechnetes Molekulargewicht 96 kDa
GenBank-ZugangsnummerBC054500
Gene symbol SEMA4D/CD100
Gene ID (NCBI) 10507
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.