CD100 Rekombinanter Antikörper

CD100 Rekombinant Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241708D10

Kat-Nr. : 84465-4-RR

Synonyme

SEMA4D, SEM4D, CD100 antigen, CD 100, A8

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  IF Staining of Jurkat using 84465-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD100 antibody (84465-4-RR, Clone: 241708D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84465-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84465-4-RR against Human CD100 were performed. The affinity constant is 3.21 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84465-4-RR bindet in IF/ICC, ELISA CD100 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
• Berechnetes Molekulargewicht: 96 kDa
• GenBank-Zugangsnummer: BC054500
• Gene symbol: SEMA4D/CD100
• Gene ID (NCBI): 10507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD100 (also known as SEMA4D), a 150 kDa homodimer, belongs to the family of immune semaphorins, and CD100 is abundantly expressed on resting T cells, NK cells, and antigen-presenting cells. As a transmembrane glycoprotein, it is digested to its soluble form, sCD100, by specific matrix metalloproteinases. CD100 has been reported that the expression levels of CD100, and one of its receptors, plexin-B1 (PLXNB1), are increased in head and neck, prostate, colon, breast, and lung cancers, and the interaction between them provides oncogenic signaling essential for tumor growth and metastasis.