CD107a / LAMP1 Monoklonaler Antikörper

CD107a / LAMP1 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

PE Fluorescent Dye

CloneNo.

H4A3

Kat-Nr. : PE-65051

Synonyme

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, H4A3

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  IF Staining of HeLa using PE-65051

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PE-Anti-Human CD107a antibody (PE-65051, Clone: H4A3) at dilution of 1:50.

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  FC experiment of HeLa using PE-65051

  1X10^6 HeLa cells were intracellularly stained with 10 ul PE Anti-Human CD107a (PE-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Produktinformation

PE-65051 bindet in IF/ICC, FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC006345
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.1% sodium azide and a stabilizer
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).