CD107a / LAMP1 Polyklonaler Antikörper

CD107a / LAMP1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32435-1-AP

Synonyme

CD107a, Lamp1, Lamp 1

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  WB analysis using 32435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 32435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 32435-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Lamp1 antibody (32435-1-AP) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C6-Zellen, Rattennierengewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32435-1-AP bindet in WB, IF/ICC, ELISA CD107a / LAMP1 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD107a / LAMP1 fusion protein Ag34623
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 90-120 kDa
• GenBank-Zugangsnummer: NM_001317353
• Gene symbol: CD107a
• Gene ID (NCBI): 16783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.