CD107a / LAMP1 Rekombinanter Antikörper

CD107a / LAMP1 Rekombinant Antikörper für IF/ICC, FC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF/ICC, FC, FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

H4A3

Kat-Nr. : 65596-1-MR

Synonyme

CD107a, LAMP1, CD107 antigen-like family member A, CD107a/LAMP1, H4A3

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  IF Staining of HepG2 using 65596-1-MR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CD107a / LAMP1 antibody (65596-1-MR, Clone: H4A3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 65596-1-MR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG2a Recombinant Antibody (65596-1-MR, Clone: H4A3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human peripheral blood platelets using 65596-1-MR

  1x10^6 thrombin-treated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG2a RecAb (65596-1-MR, Clone: H4A3) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). 1x10^6 untreated human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG2a RecAb (65596-1-MR, Clone: H4A3) and PE-conjugated Goat Anti-Mouse IgG (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen
• Erfolgreiche Detektion in FC: Thrombin treated human peripheral blood platelets

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65596-1-MR bindet in IF/ICC, FC, FC (Intra) CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC006345
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).