CD107b / LAMP2 Monoklonaler Antikörper

CD107b / LAMP2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

ABL-93

Kat-Nr. : CL488-65052

Synonyme

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3



Geprüfte Anwendungen

Erfolgreiche Detektion in IFNIH/3T3-Zellen
Erfolgreiche Detektion in FCNIH/3T3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65052 bindet in IF, FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Ratte / IgG2a, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Gereinigte Glykoproteine einer BALB/c-Mausembryo-3T3-Zelllinie
Vollständiger Name lysosomal-associated membrane protein 2
GenBank-ZugangsnummerBC138718
Gene symbol Lamp2
Gene ID (NCBI) 16784
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 CD107b / LAMP2 antibody CL488-65052Protokoll herunterladen
Standard-Protokolle
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