CD107b / LAMP2 Rekombinanter Antikörper

CD107b / LAMP2 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

H4B4

Kat-Nr. : FITC-65520

Synonyme

CD107b, LAMP2, CD107 antigen-like family member B, H4B4, LAMP 2

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  FC experiment of human PBMCs using FITC-65520

  1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD107b Rabbit Recombinant Antibody (FITC-65520, Clone:H4B4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using FITC-65520

  1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD107b Rabbit Recombinant Antibody (FITC-65520, Clone:H4B4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using FITC-65520

  1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD107b Rabbit Recombinant Antibody (FITC-65520, Clone:H4B4) or 5 ul FITC Plus Anti-Human CD107b Mouse Monoclonal Antibody (FITC-65053, Clone:H4B4). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65520 bindet in FC (Intra) CD107b / LAMP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane adhärente periphere Blutzellen
• Vollständiger Name: lysosomal-associated membrane protein 2
• Berechnetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002965
• Gene symbol: LAMP2
• Gene ID (NCBI): 3920
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).