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CD107b / LAMP2 Rekombinanter Antikörper

CD107b / LAMP2 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241893G7

Kat-Nr. : 84474-3-PBS

Synonyme

CD107b, Lamp2, 241893G7, Lamp 2, Mac3

Filter:
  • Western Blot (WB) analysis of various lysates using CD107b / LAMP2 Recombinant antibody (84474-3-RR)

    WB analysis using 84474-3-RR (same clone as 84474-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84474-3-RR (CD107b / LAMP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84474-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD107b / LAMP2 Recombinant antibody (84474-3-RR)

    IHC staining of mouse liver using 84474-3-RR (same clone as 84474-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-3-RR (CD107b / LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84474-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CD107b / LAMP2 Recombinant antibody (84474-3-RR)

    IF Staining of NIH/3T3 using 84474-3-RR (same clone as 84474-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (84474-3-RR, Clone: 241893G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84474-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84474-3-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD107b / LAMP2 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD107b / LAMP2 fusion protein Eg2029
Vollständiger Name lysosomal-associated membrane protein 2
Berechnetes Molekulargewicht46 kDa
Beobachtetes Molekulargewicht90 kDa
GenBank-ZugangsnummerNM_001017959.2
Gene symbol CD107b / LAMP2
Gene ID (NCBI) 16784
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.