CD109 Monoklonaler Antikörper

CD109 Monoklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

2H8G7

Kat-Nr. : 60179-1-Ig

Synonyme

CD109, CD109 antigen, CD109 molecule, CPAMD7, p180, Platelet specific Gov antigen, r150, RP11 525G3.1

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 60179-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60179-1-Ig (CD109 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 60179-1-Ig

  1X10^6 Jurkat cells were stained with 0.20ug CD109 antibody (60179-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

60179-1-Ig bindet in WB, IHC, FC, ELISA CD109 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CD109 molecule
• Berechnetes Molekulargewicht: 162 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: BC152996
• Gene symbol: CD109
• Gene ID (NCBI): 135228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD109, also named as CPAMD7, is a member of the alpha2-macroglobulin/complement superfamily. It is a glycosylphosphatidylinositol (GPI)-linked cell surface glycoprotein of approximately 170kd which is found on the cell surface of platelets, activated T-cells, and endothelial cells. CD109 binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). CD109 has been identified as part of the TGF-beta receptor system in human keratinocytes. Up regulation of CD109 expression has been observed in several different types of tumor.CD109 is a glycosyl-phosphatidylinositol-anchored glycoprotein that is a member of the a2-macroglobulin/C3, C4, C5 family of thioester-containing proteins. There're 2 forms of 150 (p150) and 120 kDa (p120) exist due to proteolytic degradation from a 180 kDa form. The antibody can recognize all these 4 isoforms.

Publikationen

Species	Application	Title
human	WB	Clin Oral Implants Res; Characterization of macrophages infiltrating peri-implantitis lesions.; Authors - Tobias Fretwurst
		Drug Dev Res; Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression.; Authors - Junyu Fan
human	IHC	Mol Carcinog; Tandem mass tag (TMT) quantitative protein analysis-based proteomics and parallel reaction monitoring (PRM) validation revealed that MST4 accelerates osteosarcoma proliferation by increasing MRC2 activity; Authors - Yunyan Liu