CD117 Monoklonaler Antikörper

CD117 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

2B8

Kat-Nr. : APC-65054

Synonyme

Bs, c KIT, CD117, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, Kit, kit oncogene, SCO1, SCO5, SOW3, Ssm, Tr kit

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Galerie der Validierungsdaten

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  FC experiment of mouse bone marrow cells using APC-65054

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC Anti-Mouse CD45R (B220) (FITC-65139, Clone: RA3-6B2) and 0.20 ug APC Anti-Mouse CD117 (APC-65054, Clone: 2B8). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using APC-65054

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC Anti-Mouse CD45R (B220) (FITC-65139, Clone: RA3-6B2) and 0.20 ug APC-Rat IgG2b isotype control antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65054 bindet in FC CD117 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus IL-3-abhängige Knochenmark-Mastzellen
• Vollständiger Name: kit oncogene
• GenBank-Zugangsnummer: BC052457
• Gene symbol: Kit
• Gene ID (NCBI): 16590
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und einem Stabilisator.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).