CD117/c-Kit Rekombinanter Antikörper

CD117/c-Kit Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241313C5

Kat-Nr. : 84259-5-RR

Synonyme

CD117, KIT, EC:2.7.10.1, c-Kit, C Kit

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  WB analysis of Mo7e using 84259-5-RR

  Mo7e cells were subjected to SDS PAGE followed by western blot with 84259-5-RR (CD117/c-Kit antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84259-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84259-5-RR (CD117/c-Kit antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Mo7e using 84259-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Mo7e cells using CD117/c-Kit antibody (84259-5-RR, Clone: 241313C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84259-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84259-5-RR against Human CD117/c-Kit were performed. The affinity constant is 0.90 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mo7e cells
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Mo7e cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84259-5-RR bindet in WB, IHC, IF/ICC, ELISA CD117/c-Kit und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 976 aa, 110 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC071593
• Gene symbol: CD117
• Gene ID (NCBI): 3815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early hemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).