CD117/c-Kit Rekombinanter Antikörper

CD117/c-Kit Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

241313C5

Kat-Nr. : FITC-98377

Synonyme

CD117, KIT, C Kit, c-Kit, EC:2.7.10.1

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  FC experiment of human blood cells using FITC-98377

  1x10^6 human blood cells were surface stained with CoraLite® Plus 647 Anti-Human CD34, and 5 ul FITC Plus Anti-Human C-Kit Rabbit RecAb (FITC-98377, Clone: 241313C5) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human blood cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-98377 bindet in FC CD117/c-Kit und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Berechnetes Molekulargewicht: 976 aa, 110 kDa
• GenBank-Zugangsnummer: BC071593
• Gene symbol: CD117
• Gene ID (NCBI): 3815
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).