CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(56)

CloneNo.

1C7C2

Kat-Nr. : 66519-1-Ig

Synonyme

1C7C2, CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR 3 alpha chain, CR-3 alpha chain



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTF-1-Zellen, humanes Knochenmarkgewebe
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Tonsillitisgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66519-1-Ig bindet in WB, IHC, IF-P, ELISA CD11b und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name integrin, alpha M (complement component 3 receptor 3 subunit)
Berechnetes Molekulargewicht 93 kDa
Beobachtetes Molekulargewicht 160 kDa
GenBank-ZugangsnummerNM_000632
Gene symbol CD11b
Gene ID (NCBI) 3684
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CD11b antibody 66519-1-IgProtokoll herunterladen
IHC protocol for CD11b antibody 66519-1-IgProtokoll herunterladenl
IF protocol for CD11b antibody 66519-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Cell Rep Med

Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity

Authors - Xingyu Zhou
IF

J Exp Med

TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.

Authors - Dehong Yan
humanIF

Cell Rep

Inflammatory dendritic cells restrain CD11b+CD4+ CTLs via CD200R in human NSCLC

Authors - Mingjie Lin

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
IF

Apoptosis

ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments

Authors - Qian Yan
IF

Life Sci

Urine-derived stem cells from patients alleviate lupus nephritis via regulating macrophage polarization in a CXCL14-dependent manner

Authors - Yunfei Xia

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Reyes (Verified Customer) (09-05-2024)

CD11b did not work as good as expected, not showing my microglia, but rather appearing in some small neurons, and most of the marking seems to be autofluorescence from the human brain tissue.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
CD11b Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:66519-1-Ig)
FH

Santhosh (Verified Customer) (04-15-2019)

Human FFPE tonsil tissue, HIER pH6

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
CD11b Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:66519-1-Ig)