CD11b Monoklonaler Antikörper
CD11b Monoklonal Antikörper für WB, IHC, IF-P, ELISA
Wirt / Isotyp
Maus / IgG2b
Getestete Reaktivität
human
Anwendung
WB, IHC, IF-P, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C7C2
Kat-Nr. : 66519-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | TF-1-Zellen, humanes Knochenmarkgewebe |
Erfolgreiche Detektion in IHC | humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | humanes Tonsillitisgewebe |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 20 publications below |
IHC | See 7 publications below |
IF | See 27 publications below |
Produktinformation
66519-1-Ig bindet in WB, IHC, IF-P, ELISA CD11b und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Maus / IgG2b |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Peptid |
Vollständiger Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
Berechnetes Molekulargewicht | 93 kDa |
Beobachtetes Molekulargewicht | 160 kDa |
GenBank-Zugangsnummer | NM_000632 |
Gene symbol | CD11b |
Gene ID (NCBI) | 3684 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for CD11b antibody 66519-1-Ig | Protokoll herunterladen |
IHC protocol for CD11b antibody 66519-1-Ig | Protokoll herunterladenl |
IF protocol for CD11b antibody 66519-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Rep Med Microneedle delivery of CAR-M-like engineered macrophages alleviates intervertebral disc degeneration through enhanced efferocytosis capacity | ||
J Exp Med TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis. | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Apoptosis ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments | ||
Life Sci Urine-derived stem cells from patients alleviate lupus nephritis via regulating macrophage polarization in a CXCL14-dependent manner |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (09-05-2024) | CD11b did not work as good as expected, not showing my microglia, but rather appearing in some small neurons, and most of the marking seems to be autofluorescence from the human brain tissue.
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FH Santhosh (Verified Customer) (04-15-2019) | Human FFPE tonsil tissue, HIER pH6
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