CD11a Rekombinanter Antikörper

CD11a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

TS2/4

Kat-Nr. : FITC-65518

Synonyme

CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L, ITGAL, Leukocyte adhesion glycoprotein LFA-1 alpha chain

---

• 

  FC experiment of human PBMCs using FITC-65518

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11a Rabbit Recombinant Antibody (FITC-65518, Clone:TS2/4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65518

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11a Rabbit Recombinant Antibody (FITC-65518, Clone:TS2/4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65518

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD11a Rabbit Recombinant Antibody (FITC-65518, Clone:TS2/4) (left) or 5 ul FITC Plus Anti-Human CD11a Mouse Monoclonal Antibody (FITC-65194, Clone:TS2/4) (right). Cells were not fixed. Lymphocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65518 bindet in FC CD11a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HLA-DR CTL-Linie
• Vollständiger Name: integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Berechnetes Molekulargewicht: 129 kDa
• GenBank-Zugangsnummer: BC008777
• Gene symbol: CD11a
• Gene ID (NCBI): 3683
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).