CD11b Rekombinanter Antikörper

CD11b Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

ICRF44

Kat-Nr. : 65582-1-PBS

Synonyme

ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha

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  FC experiment of human PBMCs using 65582-1-MR (same clone as 65582-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone:ICRF44) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65582-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65582-1-MR (same clone as 65582-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody (65582-1-MR, Clone:ICRF44) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65582-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65582-1-PBS bindet in FC CD11b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rheumatoide Synovialzellen und humane Monozyten
• Vollständiger Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Berechnetes Molekulargewicht: 1152 aa, 127 kDa
• GenBank-Zugangsnummer: BC096346
• Gene symbol: CD11b
• Gene ID (NCBI): 3684
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).