CD11b/c Rekombinanter Antikörper

CD11b/c Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Biotin

CloneNo.

OX42

Kat-Nr. : Biotin-65606

Synonyme

CD11b, integrin alpha M, Itgam, OX42

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  FC experiment of rat bone marrow cells using Biotin-65606

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug Biotin Anti-Rat CD11b/c (OX42) Mouse IgG2a RecAb (Biotin-65606, Clone: OX42) and CoraLite®488-conjugated streptavidin, or unstained. Cells were not fixed.

• 

  FC experiment of rat bone marrow cells using Biotin-65606

  1x10^6 rat bone marrow cells were surface stained with 0.25 ug Biotin Anti-Rat CD11b/c (OX42) Mouse IgG2a RecAb (Biotin-65606, Clone: OX42) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: rat bone marrow cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-65606 bindet in FC CD11b/c und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rat peritoneal macrophages
• Vollständiger Name: integrin alpha M
• Berechnetes Molekulargewicht: 127 kDa
• GenBank-Zugangsnummer: NM_012711
• Gene symbol: CD11b
• Gene ID (NCBI): 25021
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).