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CD11b Monoklonaler Antikörper

CD11b Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(3)

CloneNo.

OX42

Kat-Nr. : FITC-65229

Synonyme

integrin alpha M, Itgam, OX42

Filter:
  • Flow cytometry (FC) experiment of rat bone marrow cells using FITC Plus Anti-Rat CD11b (OX42) (FITC-65229)

    FC experiment of rat bone marrow cells using FITC-65229

    1x10^6 rat bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Rat CD11b/c (OX42) (FITC-65229, Clone: OX42) or 0.25 ug FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were not fixed.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCrat bone marrow cells

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

FCSee 3 publications below

Produktinformation

FITC-65229 bindet in FC CD11b und zeigt Reaktivität mit Ratten

Getestete Reaktivität Ratte
In Publikationen genannte ReaktivitätRatte
Wirt / Isotyp Maus / IgG2a, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Rat peritoneal macrophages
Vollständiger Name integrin alpha M
Berechnetes Molekulargewicht127 kDa
GenBank-ZugangsnummerNM_012711
Gene symbol CD11b
Gene ID (NCBI) 25021
Konjugation FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b (integrin alpha M or CR3A) and CD11c (integrin alpha X) are members of the integrin alpha chain family and can form α/β heterodimers with CD18 (integrin β2). CD11b/CD18 is a receptor for the complement protein fragment C3bi, and is also a receptor for fibrinogen, factor X, and ICAM1 (PMID: 2971974; 15485828). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533). The OX-42 antibody recognizes a common epitope shared between CD11b and CD11c. It binds to C3bi receptor and is known to block C3bi binding (PMID: 3512425; 8449218).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for FITC Plus CD11b antibody FITC-65229Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
ratFC

Mol Med Rep

Discovery of microglia gonadotropin‑releasing hormone receptor and its potential role in polycystic ovarian syndrome

Authors - Fan Wang
View Article
ratFC

Biomater Res

CD62E- and ROS-Responsive ETS Improves Cartilage Repair by Inhibiting Endothelial Cell Activation through OPA1-Mediated Mitochondrial Homeostasis

Authors - Pengcheng Tu
View Article
FC

Heliyon

Peimine promotes microglial polarization to the M2 phenotype to attenuate drug-resistant epilepsy through suppressing the TLR4/NF-κB/HIF-1α signaling pathway in a rat model and in BV-2 microglia

Authors - Chongchong Liu
View Article