CD11c Rekombinanter Antikörper

CD11c Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

BU15

Kat-Nr. : 65600-1-PBS

Synonyme

Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

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  FC experiment of human peripheral blood leukocyte using 65600-1-MR (same clone as 65600-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65600-1-MR, Clone: BU15) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65600-1-PBS in a different storage buffer formulation.

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  FC experiment of human peripheral blood leukocyte using 65600-1-MR (same clone as 65600-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65600-1-MR, Clone: BU15) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65600-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocyte using 65600-1-MR (same clone as 65600-1-PBS)

  1x10^6 human peripheral blood leukocyte were surface stained with 0.25 ug Anti-Human CD11c Mouse IgG2a Recombinant Antibody (65600-1-MR, Clone: BU15) (left) or 0.25 ug Anti-Human CD11c (65196-1-Ig, Clone: BU15) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65600-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65600-1-PBS bindet in FC CD11c und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane dendritische Zellen aus Synovialflüssigkeit
• Vollständiger Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Berechnetes Molekulargewicht: 1169 aa, 129 kDa
• GenBank-Zugangsnummer: BC038237
• Gene symbol: CD11c
• Gene ID (NCBI): 3687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).