CD11c Monoklonaler Antikörper

CD11c Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F1C10

Kat-Nr. : 60258-1-PBS

Synonyme

2F1C10, CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX

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  WB analysis of HL-60 using 60258-1-Ig (same clone as 60258-1-PBS)

  Untreated and PNGase F-treated lysates of HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 60258-1-Ig (same clone as 60258-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 60258-1-Ig (same clone as 60258-1-PBS)

  Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 60258-1-Ig (same clone as 60258-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 60258-1-Ig (same clone as 60258-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60258-1-Ig (same clone as 60258-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60258-1-Ig (CD11c/Integrin Alpha X antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60258-1-Ig (CD11c/Integrin alpha X antibody) at dilution of 1:25600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human appendicitis using 60258-1-Ig (same clone as 60258-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CD11c/Integrin Alpha X antibody (60258-1-Ig, Clone: 2F1C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60258-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60258-1-PBS bindet in WB, IHC, IF-P, ELISA CD11c und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD11c fusion protein Ag11350
• Vollständiger Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Berechnetes Molekulargewicht: 1169 aa, 129 kDa
• Beobachtetes Molekulargewicht: 145 kDa
• GenBank-Zugangsnummer: BC038237
• Gene symbol: CD11c
• Gene ID (NCBI): 3687
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.