CD122/IL-2RB Rekombinanter Antikörper

CD122/IL-2RB Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241677H7

Kat-Nr. : 98151-1-RR

Synonyme

IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta

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  FC experiment of human PBMCs using 98151-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (98151-1-RR, Clone: 241677H7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56 Rabbit Recombinant Antibody (CL647-98033, Clone: 240078A7). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98151-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD122/IL-2RB Rabbit Recombinant Antibody (98151-1-RR, Clone: 241677H7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 98151-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98151-1-RR against Human CD122/IL-2RB were performed. The affinity constant is 0.645 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98151-1-RR bindet in FC CD122/IL-2RB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 2 receptor, beta
• Berechnetes Molekulargewicht: 551 aa, 61 kDa
• GenBank-Zugangsnummer: BC025691
• Gene symbol: IL2RB
• Gene ID (NCBI): 3560
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor-mediated endocytosis and transduces the mitogenic signals of interleukin 2.