CD126/IL-6R alpha Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD126/IL-6R alpha Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23457-1-PBS

Synonyme

CD126, IL-6R alpha, gp80, IL 6 receptor subunit alpha, IL 6R 1

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  WB analysis using 23457-1-AP (same clone as 23457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23457-1-AP (same clone as 23457-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23457-1-AP (IL-6R alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  IP result of anti-CD126/IL-6R alpha (IP:23457-1-AP, 4ug; Detection:23457-1-AP 1:3000) with mouse spleen tissue lysate 2480 ug. This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 23457-1-AP (same clone as 23457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 23457-1-AP (CD126/IL-6R alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23457-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23457-1-PBS bindet in WB, IHC, IP, Indirect ELISA CD126/IL-6R alpha und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD126/IL-6R alpha fusion protein Ag18263
• Vollständiger Name: interleukin 6 receptor
• Berechnetes Molekulargewicht: 468 aa, 52 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC132684
• Gene symbol: IL-6R alpha
• Gene ID (NCBI): 3570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).