CD127 (IL-7Ra) Monoklonaler Antikörper

CD127 (IL-7Ra) Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

Publikationen

1

CloneNo.

A7R34

Kat-Nr. : PE-65093

Synonyme

CD127, IL 7Ralpha, IL- 7Ralpha, Il7r, IL-7Ra, interleukin 7 receptor

---

Galerie der Validierungsdaten

• 

  FC experiment of mouse splenocytes using PE-65093

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug APC-Anti-Mouse CD3 (APC-65077, clone 17A2) and 0.25 ug PE-Anti-Mouse CD127 (IL-7Ra) (PE-65093, clone A7R34). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65093

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug APC-Anti-Mouse CD3 (APC-65077, clone 17A2) and 0.25 ug PE-rat IgG2a isotype control. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

PE-65093 bindet in FC CD127 (IL-7Ra) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: IL-7Ra-IgG1-Fusionsprotein
• Vollständiger Name: interleukin 7 receptor
• GenBank-Zugangsnummer: BC089571
• Gene symbol: Il7r
• Gene ID (NCBI): 16197
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).

Publikationen

Species	Application	Title
mouse	FC	J Inflamm Res; Sodium Butyrate Ameliorates Type 2 Diabetes-Related Sarcopenia Through IL-33-Independent ILC2s/IL-13/STAT3 Signaling Pathway; Authors - Yuan Cao