CD13 Rekombinanter Antikörper

CD13 Rekombinant Antikörper für IF/ICC, FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

CloneNo.

WM15

Kat-Nr. : 65633-1-MR

Synonyme

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M

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  IF Staining of THP-1 using 65633-1-MR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65633-1-MR, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of human PBMCs using 65633-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a RecAb (65633-1-MR, Clone:WM15) and PE-conjugated Goat Anti-Mouse IgG, or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human PBMCs using 65633-1-MR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD13 (WM15) Mouse IgG2a RecAb (65633-1-MR, Clone: WM15) (red) and PE-conjugated Goat Anti-Mouse IgG, or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65633-1-MR bindet in IF/ICC, FC CD13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane AML-Zellen
• Vollständiger Name: alanyl (membrane) aminopeptidase
• Berechnetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC058928
• Gene symbol: CD13
• Gene ID (NCBI): 290
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane protein expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)