CD13 Rekombinanter Antikörper

CD13 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Biotin

CloneNo.

WM15

Kat-Nr. : Biotin-65633

Synonyme

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M

---

• 

  FC experiment of human PBMCs using Biotin-65633

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD13 (WM15) Mouse IgG2a RecAb (Biotin-65633, Clone: WM15) and CoraLite®488-conjugated streptavidin, or unstained. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65633

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Plus Anti-Human CD13 (WM15) Mouse IgG2a RecAb (Biotin-65633, Clone: WM15) (red) and CoraLite®488-conjugated streptavidin, or unstained (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-65633 bindet in FC CD13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane AML-Zellen
• Vollständiger Name: alanyl (membrane) aminopeptidase
• Berechnetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC058928
• Gene symbol: CD13
• Gene ID (NCBI): 290
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane protein expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)