CD134 Rekombinanter Antikörper

CD134 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Biotin

CloneNo.

OX-86

Kat-Nr. : Biotin-FcA65657

Synonyme

Tnfrsf4, Ox40, OX40 antigen, OX40L receptor, Tumor necrosis factor receptor superfamily member 4

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  FC experiment of mouse splenocytes using Biotin-FcA65657

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD134 (OX-86) Rabbit IgG RecAb (Biotin-FcA65657, Clone: OX-86) and PE Streptavidin (Cat.No. PF00021). Cells were then stained with FcZero-rAb™ APC Anti-Mouse CD3e (145-2C11) Rabbit IgG Recombinant Antibody (APC-FcA65651, Clone: 145-2C11). Cells were not fixed.

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  FC experiment of mouse splenocytes using Biotin-FcA65657

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CD134 (OX-86) Rabbit IgG RecAb (Biotin-FcA65657, Clone: OX-86) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and PE Streptavidin (Cat.No. PF00021). 1x10^6 untreated mouse splenocytes surface stained with 0.25 ug Biotin Anti-Mouse CD134 (OX-86) Rabbit IgG RecAb (Biotin-FcA65657, Clone: OX-86) (black, dashed) and PE Streptavidin (Cat.No. PF00021). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-FcA65657 bindet in FC CD134 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 4
• GenBank-Zugangsnummer: BC065782
• Gene symbol: CD134
• Gene ID (NCBI): 22163
• Konjugation: Biotin
• Excitation/Emission maxima wavelengths: -
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).