CD134/OX40 Rekombinanter Antikörper

CD134/OX40 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241398G7

Kat-Nr. : 98181-1-RR

Synonyme

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

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  FC experiment of human PBMCs using 98181-1-RR

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD4 (RPA-T4) (APC-65143, Clone: RPA-T4). Cells were not fixed.

• 

  FC experiment of human PBMCs using 98181-1-RR

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) (red) or Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD134/OX40 Rabbit Recombinant Antibody (98181-1-RR, Clone: 241398G7) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98181-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98181-1-RR against Human CD134/OX40 were performed. The affinity constant is 5.48 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98181-1-RR bindet in FC CD134/OX40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 4
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_003327
• Gene symbol: CD134
• Gene ID (NCBI): 7293
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).