CD134/OX40 Rekombinanter Antikörper

CD134/OX40 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242901A7

Kat-Nr. : 98409-2-RR

Synonyme

CD134, Tnfrsf4, Ox40, OX40 antigen, OX40L receptor

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  FC experiment of BALB/c mouse splenocytes using 98409-2-RR

  1x10^6 Untreated or CD3/CD28 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5). Cells were not fixed.

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  FC experiment of BALB/c mouse splenocytes using 98409-2-RR

  1x10^6 CD3/CD28 treated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). 1x10^6 untreated BALB/c mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD134/OX40 Rabbit RecAb (98409-2-RR, Clone:242901A7) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: CD3/CD28 treated BALB/c mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98409-2-RR bindet in FC CD134/OX40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD134/OX40 fusion protein Eg2936
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 4
• Berechnetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_011659.2
• Gene symbol: CD134
• Gene ID (NCBI): 22163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).