CD134/OX40 Rekombinanter Antikörper

CD134/OX40 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241398G7

Kat-Nr. : CL647-98181

Synonyme

TNFRSF4, 241398G7, ACT35, ACT35 antigen, CD134

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  FC experiment of human PBMCs using CL647-98181

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4 and 5 ul CoraLite® Plus 647 Anti-Human CD134/OX40 Rabbit RecAb (CL647-98181, Clone: 241398G7). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-98181

  1x10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD134/OX40 Rabbit RecAb (CL647-98181, Clone: 241398G7) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD134/OX40 Rabbit RecAb (CL647-98181, Clone: 241398G7) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA-treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-98181 bindet in FC CD134/OX40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD134/OX40 fusion protein Eg1261
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 4
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_003327
• Gene symbol: CD134
• Gene ID (NCBI): 7293
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).