CD138/Syndecan-1 Polyklonaler Antikörper

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• KD/KO Validated

CD138/Syndecan-1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10593-1-PBS

Synonyme

CD138, SDC, SDC1, SYND1, syndecan

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  WB analysis using 10593-1-AP (same clone as 10593-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10593-1-AP (same clone as 10593-1-PBS)

  WB result of CD138/Syndecan-1 antibody (10593-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD138/Syndecan-1 transfected HeLa cells. This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 10593-1-AP (same clone as 10593-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 10593-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10593-1-PBS bindet in WB, IHC, Indirect ELISA CD138/Syndecan-1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Vollständiger Name: syndecan 1
• Berechnetes Molekulargewicht: 32.5 kDa
• Beobachtetes Molekulargewicht: 60-100 kDa, 130 kDa
• GenBank-Zugangsnummer: BC008765
• Gene symbol: CD138
• Gene ID (NCBI): 6382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.