CD138/Syndecan-1 Monoklonaler Antikörper

CD138/Syndecan-1 Monoklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

4F3A8

Kat-Nr. : 67155-1-Ig

Synonyme

CD138, 4F3A8, syndecan, SYND1, SDC1

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  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 67155-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67155-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 67155-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below

Produktinformation

67155-1-Ig bindet in IHC, IF-P, ELISA CD138/Syndecan-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Vollständiger Name: syndecan 1
• Berechnetes Molekulargewicht: 32.5 kDa
• GenBank-Zugangsnummer: BC008765
• Gene symbol: CD138
• Gene ID (NCBI): 6382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

Publikationen

Species	Application	Title
human	IF	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	IF	Nat Commun; Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection; Authors - Pei-Fen Zhang
human	IF	Dev Cell; SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements; Authors - Yanglin Chen
human	IF	Transl Cancer Res; By integrating single-cell RNA sequencing and bulk RNA sequencing, plasma cells signature and tertiary lymphoid structures were verified to contribute to outcome in lung adenocarcinoma; Authors - Zetian Gong