CD138/Syndecan-1 Monoklonaler Antikörper

CD138/Syndecan-1 Monoklonal Antikörper für IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

4F3A8

Kat-Nr. : 67155-1-PBS

Synonyme

CD138, 4F3A8, SDC, SDC1, SYND1

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  Sandwich ELISA standard curve of MP50044-1

  Sandwich ELISA standard curve of MP50044-1, SDC1,CD138 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60185-1-PBS. Detection antibody: 67155-1-PBS. Standard: Ag17733. Range: 0.188-12 ng/mL.

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  Sample test of MP50044-1

  Plasma of sixteen individual healthy human donors was measured. The CD138/Syndecan-1 concentration of detected samples was determined to be 293.79 ng/mL with a range of 89.65-474.60 ng/mL.

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  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (SDC1,CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67155-1-Ig (same clone as 67155-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67155-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67155-1-PBS bindet in IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test CD138/Syndecan-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Vollständiger Name: syndecan 1
• Berechnetes Molekulargewicht: 32.5 kDa
• GenBank-Zugangsnummer: BC008765
• Gene symbol: CD138
• Gene ID (NCBI): 6382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.