CD138/Syndecan-1 Rekombinanter Antikörper

CD138/Syndecan-1 Rekombinant Antikörper für IHC, FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, FC

Konjugation

Unkonjugiert

CloneNo.

240422C4

Kat-Nr. : 98051-1-RR

Synonyme

CD138, 240422C4, syndecan, SYND1, SDC1

---

• 

  IHC staining of human tonsillitis using 98051-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 98051-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of U266 using 98051-1-RR

  1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody (98051-1-RR, Clone: 240422C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98051-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98051-1-RR against Human CD138/Syndecan-1 were performed. The affinity constant is 20.8 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: U266 cells

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

98051-1-RR bindet in IHC, FC CD138/Syndecan-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: syndecan 1
• Berechnetes Molekulargewicht: 32.5 kDa
• GenBank-Zugangsnummer: BC008765
• Gene symbol: CD138
• Gene ID (NCBI): 6382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.