CD14 Monoklonaler Antikörper

CD14 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C1D9

Kat-Nr. : 60253-1-PBS

Synonyme

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 2C1D9

---

• 

  WB analysis of THP-1 using 60253-1-Ig (same clone as 60253-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60253-1-Ig (same clone as 60253-1-PBS)

  Untreated and PNGase F-treated lysates of human milk were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60253-1-Ig (same clone as 60253-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 60253-1-Ig (CD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60253-1-Ig (CD14 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60253-1-Ig (same clone as 60253-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD14 antibody (60253-1-Ig, Clone: 2C1D9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 60253-1-Ig (same clone as 60253-1-PBS)

  Immunofluorescent analysis of RAW 264.7 cells using 60253-1-Ig (CD14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60253-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50282-2

  Cytometric bead array standard curve of MP50282-2, CD14 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60253-1-PBS. Detection antibody: 60253-2-PBS. Standard:Ag10693. Range: 1.563-200 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

60253-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA CD14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD14 fusion protein Ag10693
• Vollständiger Name: CD14 molecule
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC010507
• Gene symbol: CD14
• Gene ID (NCBI): 929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.