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CD14 Rekombinanter Antikörper

CD14 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240225C2

Kat-Nr. : 83297-8-PBS

Synonyme

CD14 antigen, 240225C2

Filter:
  • Western Blot (WB) analysis of RAW 264.7 cells using CD14 Recombinant antibody (83297-8-RR)

    WB analysis of RAW 264.7 using 83297-8-RR (same clone as 83297-8-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83297-8-RR (CD14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83297-8-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using CD14 Recombinant antibody (83297-8-RR)

    IF Staining of RAW 264.7 using 83297-8-RR (same clone as 83297-8-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (83297-8-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83297-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83297-8-RR

    Affinity and Kinetic Characterization of 83297-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83297-8-RR against Mouse CD14 were performed. The affinity constant is 0.219 nM.

Geprüfte Anwendungen

Produktinformation

83297-8-PBS bindet in WB, IF/ICC, ELISA CD14 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name CD14 antigen
Berechnetes Molekulargewicht39 kDa
Beobachtetes Molekulargewicht45-55 kDa
GenBank-ZugangsnummerNM_009841.4
Gene symbol Cd14
Gene ID (NCBI) 12475
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.