CD14 Rekombinanter Antikörper

CD14 Rekombinant Antikörper für IF/ICC, FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

CloneNo.

240225C2

Kat-Nr. : 98020-1-RR

Synonyme

CD14 antigen, 240225C2

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  IF Staining of RAW 264.7 using 98020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of mouse peritoneal macrophages using 98020-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen
• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

98020-1-RR bindet in IF/ICC, FC CD14 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: CD14 antigen
• Berechnetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: NM_009841.4
• Gene symbol: Cd14
• Gene ID (NCBI): 12475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.