CD14 Rekombinanter Antikörper

CD14 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

230332D7

Kat-Nr. : 98040-1-PBS

Synonyme

230332D7, CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein

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  FC experiment of human PBMCs using 98040-1-RR (same clone as 98040-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) or 0.25 ug Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98040-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98040-1-RR (same clone as 98040-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD14 Rabbit Recombinant Antibody (98040-1-RR, Clone: 230332D7) (red) or 0.25 ug Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98040-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98040-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98040-1-RR against Human CD14 were performed. The affinity constant is 0.703 nM.

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Geprüfte Anwendungen

Produktinformation

98040-1-PBS bindet in FC CD14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD14 fusion protein Eg0498
• Vollständiger Name: CD14 molecule
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• GenBank-Zugangsnummer: BC010507
• Gene symbol: CD14
• Gene ID (NCBI): 929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.