CD14 Monoklonaler Antikörper

CD14 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Publikationen(1)

CloneNo.

UCHM-1

Rezensionen (1)

Kat-Nr. : CL647-65056

Synonyme

CD14 molecule, Monocyte differentiation antigen CD14, membrane-bound form, Monocyte differentiation antigen CD14, urinary form, Myeloid cell-specific leucine-rich glycoprotein, UCHM-1

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  FC experiment of human PBMCs using CL647-65056

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14, and 5 ul CoraLite® Plus 647 Anti-Human CD14 (UCHM-1) (CL647-65056, Clone: UCHM-1) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL647-65056

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD14 (UCHM-1) (CL647-65056, Clone: UCHM-1) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL647-65056 bindet in IF, FC CD14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Thymozyten und Lymphozyten aus peripherem Blut eines in Remission befindlichen AML-Patienten
• Vollständiger Name: CD14 molecule
• Berechnetes Molekulargewicht: 375 aa, 40 kDa
• GenBank-Zugangsnummer: BC010507
• Gene symbol: CD14
• Gene ID (NCBI): 929
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publikationen

Species	Application	Title
human	IF	Oncol Lett; Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation; Authors - Yufei Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Cyril (Verified Customer) (11-28-2024)

We tested the product on the Attune with excitation at 637 nm. I attempted to record the data on the RL1 filter (670/14), but it is only visible on RL2 (720/30). Since the cytometer is checked before each use, it is possible that the fluorochrome emits a shifted spectrum

• Applications: Flow Cytometry
• Cell Tissue Type: THP-1 and THP-1 stimulated with PMA.

CL Plus 647 CD14 Antibody Flow Cytometry validation ( dilution) in THP-1 and THP-1 stimulated with PMA. (Cat no:CL647-65056)