CD141/Thrombomodulin Rekombinanter Antikörper

CD141/Thrombomodulin Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240365D1

Kat-Nr. : CL488-83416-4

Synonyme

THBD, Thrombomodulin, 240365D1, CD141, Fetomodulin

---

• 

  IF Staining of A431 using CL488-83416-4

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 CD141/Thrombomodulin antibody (CL488-83416-4, Clone: 240365D1 ) at dilution of 1:100.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-83416-4 bindet in IF/ICC CD141/Thrombomodulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD141/Thrombomodulin fusion protein Eg0944
• Vollständiger Name: thrombomodulin
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: NM_000361.3
• Gene symbol: THBD
• Gene ID (NCBI): 7056
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).